Isolation and characterization of two YUCCA flavin monooxygenase genes from cultivated strawberry (Fragaria × ananassa Duch.)

Liu, H. and Ying, Y. and Zhang, L. and et al, . (2012) Isolation and characterization of two YUCCA flavin monooxygenase genes from cultivated strawberry (Fragaria × ananassa Duch.). Plant Cell Reports, 31 (8). pp. 1425-1435.

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In strawberry (Fragaria × ananassa Duch.), auxin has been recognized as the main signal molecule coordinating the growth and initiation of ripening of fruits. The molecular mechanism regulating auxin biosynthesis in strawberry remains unknown. This project reports two YUCCA flavin monooxygenase genes FaYUC1-2 isolated from cultivated strawberry. FaYUC1 and FaYUC2 are most homologous to AtYUC6 and AtYUC4, respectively. Significant expression of FaYUC1-2 is found in vegetative meristems and reproductive organs, with overlapping but distinct patterns. During fruit development, both transcripts of FaYUC1 and FaYUC2 in achenes reach a peak around large green fruit (G2) stage, but the sudden rise in FaYUC2 transcript level is much steeper and begins earlier than that in FaYUC1. FaYUC2 is also obviously expressed in the receptacles from green fruits, hinting another auxin source for receptacle development, other than achenes. FaYUC1 over-expression Arabidopsis exhibits typical auxin hyper-accumulation phenotype in many aspects, such as the narrow and downward curled leaves, strong apical dominance, short and hairy root. It is also severely sterile, due to the disruption of floral meristems initiation and floral organs development. Transgenic analysis indicates that strawberry YUC gene may hold conserved role in auxin biosynthesis like their homologs in other plants. Integrated with the spatiotemporal expression features, these results led us to propose that FaYUC1-2 may involve in many developmental processes including flower and fruit development in strawberry.

Item Type: Article
Additional Information: The authors would like to thank anonymous referees for comments on the manuscript. This work was supported by funds from the Science and Technology Commission of Shanghai Municipality (Shanghai Rising-Star Program, 09QA1405300) and the Agricultural Commission of Shanghai Municipality (Key Program, 2008-No.1-4).
Uncontrolled Keywords: Fragaria 9 ananassa Duch, YUCCA flavin monooxygenase, Auxin biosynthesis, Auxin hyper-accumulation
Author Affiliation: College of Horticultural Sciences, Nanjing Agricultural University, China
Subjects: Crop Improvement
Crop Improvement > Biotechnology
Divisions: Other Crops
Depositing User: Mr Arbind Seth
Date Deposited: 12 Dec 2012 09:27
Last Modified: 12 Dec 2012 09:27
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