Sugarcane genes differentially expressed in response to Puccinia melanocephala infection: identification and transcript profiling.

Oloriz, M.I. and Gil, V. and Rojas, L. (2012) Sugarcane genes differentially expressed in response to Puccinia melanocephala infection: identification and transcript profiling. Plant Cell Reports, 31 (5). pp. 955-969.

[img] PDF - Published Version
Restricted to ICRISAT researchers only


Brown rust caused by the fungus Puccinia melanocephala is a major disease of sugarcane (Saccharum spp.). A sugarcane mutant, obtained by chemical mutagenesis of the susceptible variety B4362, showed a post-haustorial hypersensitive response (HR)-mediated resistance to the pathogen and was used to identify genes differentially expressed in response to P. melanocephala via suppression subtractive hybridization (SSH). Tester cDNA was derived from the brown rust-resistant mutant after inoculation with P. melanocephala, while driver cDNAs were obtained from the non-inoculated resistant mutant and the inoculated susceptible donor variety B4362. Database comparisons of the sequences of the SSH recombinant clones revealed that, of a subset of 89 non-redundant sequences, 88% had similarity to known functional genes, while 12% were of unknown function. Thirteen genes were selected for transcript profiling in the resistant mutant and the susceptible donor variety. Genes involved in glycolysis and C4 carbon fixation were up-regulated in both interactions probably due to disturbance of sugarcane carbon metabolism by the pathogen. Genes related with the nascent polypeptide associated complex, post-translational proteome modulation and autophagy were transcribed at higher levels in the compatible interaction. Up-regulation of a putative L-isoaspartyl O-methyltransferase S-adenosylmethionine gene in the compatible interaction may point to fungal manipulation of the cytoplasmatic methionine cycle. Genes coding for a putative no apical meristem protein, S-adenosylmethionine decarboxylase, non-specific lipid transfer protein, and GDP-L-galactose phosphorylase involved in ascorbic acid biosynthesis were up-regulated in the incompatible interaction at the onset of haustorium formation, and may contribute to the HR-mediated defense response in the rust-resistant mutant.

Item Type: Article
Additional Information: The authors want to thank David De Vleesschauwer (Ghent University, Belgium) for fruitful discussions concerning qPCR experimental setup. The authors are grateful for the financial support of the Ministry of Science and Technology (CITMA,Cuba, project 300227) and the Flemish Interuniversity Council (IUC UCLV/VLIR and VLIR-UOS).
Uncontrolled Keywords: Hypersensitive response, Sugarcane brown rust, Suppression subtractive hybridization
Author Affiliation: Instituto de Biotecnología de las Plantas, Universidad Central Marta Abreu de Las Villas, Cuba.
Subjects: Crop Improvement > Genetics/Genomics
Divisions: Other Crops
Depositing User: Mr Arbind Seth
Date Deposited: 12 Dec 2012 12:05
Last Modified: 12 Dec 2012 12:05
Official URL:

Actions (login required)

View Item View Item