Peanut genes identified during initial phase of Cercosporidium personatum infection

Nobile, P.M. and Lopes, C.R. and Barsalobres-Cavallari, C. and et al, . (2008) Peanut genes identified during initial phase of Cercosporidium personatum infection. Plant Science, 174 (1). pp. 78-87.

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Late leaf spot (LLS), caused by the fungus Cercosporidium personatum, is one of the most severe diseases in peanut (Arachis hypogaea). The vast majority of commercial cultivars do not exhibit satisfactory levels of resistance to the pathogen, whereas non-commercial genotypes cv. 850 and cv. 909 are resistant to LLS and show symptoms similar to hypersensitive response (HR) lesions. In the present study, we investigated the molecular components of the initial stages of the resistance by gene expression profiling using suppression subtractive hybridization and differential screening of cDNA macroarray techniques. Gene expression analyses have allowed us to identify more than 700 peanut unique expressed sequence tags (EST) involved in several aspects of the early stages of C. personatum pathogenesis, such as components of defense signaling pathways, gene expression regulators, cell cycle controlling genes and components of the biosynthesis of transducer and antimicrobial compounds. The most significantly induced gene corresponds to a novel O′-methyltranferase, suggesting its involvement in the production of local lesions in C. personatum-resistant A. hypogea genotypes. Taken together, our results contribute to elucidate the defense strategies of peanut and provide the framework for the generation of pathogen-resistant peanut cultivars.

Item Type: Article
Additional Information: We thank Dr. J. Ignacio Godoy, Dr. Sergio de A. Moraes and Dr. Alessandra Favero for plant and fungal material and their suggestion in conducting the experiments, Dr. Tatiany Barata Pereira and Prof. Dr. Roberto Antonio Rodella for optical microscopy, Dr. Erika C. Jorge for helping and advising with the arrays, Matheus Patrıcio for the bioinformatics support, Dr. Yumi Hiraki for SSH advice and Conselho Nacional de Desenvolvimento Cientıfico e Tecnolo´gico (CNPq) for a PhD fellowship to P.M.N. This work was supported by Fundacao de Amparo a Pesquisa (FAPESP) and partially supported by Projeto de Apoio ao Desenvolvimento de Tecnologia Agropecuaria para o Brasil (PRODETAB).
Uncontrolled Keywords: Peanut, Late leaf spot, Host defense response, O-Methyltransferase, Suppression subtractive hybridization
Author Affiliation: Instituto Agronomico de Campinas – RGV, Brazil
Subjects: Crop Improvement
Crop Improvement > Genetics/Genomics
Divisions: Groundnut
Depositing User: Mr Arbind Seth
Date Deposited: 01 Jan 2013 09:14
Last Modified: 01 Jan 2013 09:14
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