Genetic variability in Macrophomina phaseolina (Tassi.) Goid., causal agent of charcoal rot of sorghum

Prabhu, H.V. and Adiver, S.S. and Bhat, R. and Narayana, Y.D. and Jahagirdar, S. and Parameshwarappa, K.G. (2012) Genetic variability in Macrophomina phaseolina (Tassi.) Goid., causal agent of charcoal rot of sorghum. Karnataka Journal of Agricultural Sciences, 25 (1). pp. 72-76.

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Thirty five isolates of M. phaseolina, causal organism of Charcoal rot of sorghum, were obtained from different agro-climatic regions of major rabi sorghum growing areas (Karnataka, Maharashtra and AndhraPradesh). Variation among different isolates was studied using PCR-RAPD markers. From a set of 20 random primers tested, a total of 142 amplicon levels were available for analysis. Primers, OPA-03 and OPA-08 showed cent per cent polymorphism. It was possible to discriminate all the isolates with any of the primers employed. UPGMA clustering of data indicated that the isolates showed varied levels of genetic similarity within a range of 0.58 to 0.97 similarity coefficient index indicating significant levels of variation. The study suggested that the grouping of isolates was not related to the regions of collection in anyway. Maximum genetic similarity of 97 % was found between Solapur and Mulegaon. Least genetic similarity was observed between Ahmednagar and Mehboobnagar isolates.

Item Type: Article
Uncontrolled Keywords: Macrophomina. phaseolina, Polymerase Chain Reaction- Random Amplified Polymorphic DNA, Rabi sorghum
Author Affiliation: Department of Plant Pathology, University of Agricultural Sciences, Dharwad- 580 005, India
Subjects: Crop Improvement
Divisions: Sorghum
Depositing User: Mr B Krishnamurthy
Date Deposited: 23 Jan 2013 14:07
Last Modified: 23 Jan 2013 14:07

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