Genetic Diversity of Fusarium oxysporum f.sp. cubense Isolates (Foc) of India by Inter Simple Sequence Repeats (ISSR) Analysis

Thangavelu, R. and Muthu Kumar, K. and Devi, P.G. and Mustaffa, M.M. (2012) Genetic Diversity of Fusarium oxysporum f.sp. cubense Isolates (Foc) of India by Inter Simple Sequence Repeats (ISSR) Analysis. Molecular Biotechnology, 51 (3). pp. 203-211.

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To find out the genetic diversity of Indian Foc isolates of banana, a total of 107 isolates of Fusarium which includes 98 Foc isolates obtained from different banana growing regions of India and seven Foc isolates belong to all known VCGs obtained from Australia and two non-pathogenic Fusarium oxysporum (npFo) isolates were subjected to ISSR analysis. In the initial screening of ISSR primers, out of 34, 10 primers which generated more polymorphic bands were selected for further analysis. The Phylogenetic analysis carried out based on the fingerprints obtained through ISSR analysis indicated the presence of wide genetic diversity among the Foc isolates of India and also its polyphyletic nature. Totally, seven different clusters were obtained and these clusters differentiated the Foc isolates of India based on the races/VCGs. Besides, the cluster analysis clearly distinguished the freshly emerged Foc strain obtained from cv. Grand Naine (Cavendish-AAA) and Poovan (Mysore-AAB) from the other Foc isolates. The non-pathogenic F. oxysporum isolates which have been included for comparison purpose also clustered separately. All these above said findings indicates for the first time the discriminatory power of ISSR to clearly distinguish and separate the Foc isolates according to its race/VCGs and also its virulence. This study would be useful not only to design and develop effective management strategies but also useful for quarantine purposes.

Item Type: Article
Additional Information: We thank Mr. V. Govindasamy, Senior Research Fellow for his technical assistance and Dr. Lisa Maree Gulino, QDPI &F, Brisbane for the supply of DNA samples of Fusarium spp.
Uncontrolled Keywords: ISSR analysis,Fusarium wilt of banana,Diversity analysis
Author Affiliation: National Research Centre for Banana, Thogamali main road, Thayanur post, Thiruchirapalli 620102, Tamil Nadu, India.
Subjects: Crop Improvement
Divisions: Other Crops
Depositing User: Mr Balakrishna Garadasu
Date Deposited: 03 Feb 2013 11:20
Last Modified: 03 Feb 2013 11:20
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