Early-generation germplasm introgression from Sorghum macrospermum into sorghum (S. bicolor)

Kuhlman, L.C. and Burson, B.L. and Stelly, D.M. and Klein, P.E. and Klein, R.R. and Price, H.J. and Rooney, W.L. (2010) Early-generation germplasm introgression from Sorghum macrospermum into sorghum (S. bicolor). Genome, 53 (6). pp. 419-429.

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Sorghum has been improved by public and private breeding programs utilizing germplasm mostly from within the species Sorghum bicolor. Recently, hybridization with an Australian species, S. macrospermum (AAB1B1YYZZ), has been demonstrated and the genomic relationship to S. bicolor (AAB1B1) shown to be partially compatible. For this species to be potentially useful to sorghum improvement programs, there must be documented introgression into an S. bicolor background. Fifteen BC1F1 progeny were recovered using the interspecific hybrid as a female and embryo rescue. In these progeny, chromosome numbers ranged from 35 to 70 and all were essentially male-sterile. Repeated backcrossing with S. bicolor pollen produced BC2F1 seed on 3 of the 15 BC1F1 plants. BC2F1 progeny had varying levels of male fertility; selfed seed set ranged from 0% to 95%, with only 2 individuals being completely male-sterile. Using AFLP and SSR markers, genomic introgression of S. macrospermum ranged from 0% to 18.6%. Cytogenetic analysis of 19 individuals revealed that chromosome numbers were 20, except for a single backcross that had 21 chromosomes. Molecular cytogenetic analysis confirmed the presence of recombinant introgression chromosomes as well as alien addition and alien substitution chromosomes within the BC2F1s.

Item Type: Article
Author Affiliation: Department of Soil Science and Crop Science, Texas A& M University, College Station TX77843, USA
Subjects: Crop Improvement > Plant Breeding
Crop Improvement > Seed Technology
Divisions: Sorghum
Depositing User: Sandhya Gir
Date Deposited: 12 Dec 2010 15:40
Last Modified: 29 Dec 2010 19:53
Official URL: http://dx.doi.org/10.1139/G10-027
URI: http://eprints.icrisat.ac.in/id/eprint/960

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