Molecular diversity and phylogeny of rhizobia associated with wild legumes native to Xinjiang, China

Han, T. X. and Wang, E. T. and Han, L. L. and et al, . (2008) Molecular diversity and phylogeny of rhizobia associated with wild legumes native to Xinjiang, China. Systematic and Applied Microbiology, 31 (4). pp. 287-301.

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A total of 111 rhizobial strains were isolated from wild legumes in Xinjiang, an isolated region of northwest China. Nine genomic species belonging to four genera of Rhizobium, Mesorhizobium, Ensifer, and Bradyrhizobium were defined among these strains based on the characterization of amplified 16S ribosomal DNA restriction analysis (ARDRA), restriction fragment length polymorphism (RFLP) analysis of 16S-23S rDNA intergenic spacers (IGS), 16S rRNA gene sequencing and multilocus sequence analysis (MLSA). Twenty-five nodC types corresponding to eight phylogenetic clades were divided by RFLP and sequence analysis of the PCR-amplified nodC gene. The acid-producing Rhizobium and Mesorhizobium species were predominant, which may be related to both the local environments and the hosts sampled. The present study also showed the limitation of using nod genes to estimate the host specificity of rhizobia.

Item Type: Article
Additional Information: We thank Prof. Fu Suo Zhang for providing fund for nodule collection and Prof. Ge Hong Wei for providing the seeds of some wild leguminous plants. We are also very grateful to the anonymous referee for his (or her) valuable comments. This work was supported by the National Basic Research Program of China (2006CB100206), the National Program for Basic S and T Platform Construction (2005DKA21201-10), and the National Natural Science Foundation of China (30670001 and 30400001).
Uncontrolled Keywords: Xinjiang, Wild legumes, Rhizobial diversity, Phylogeny, MLSA
Author Affiliation: Key Laboratory of Agro-Microbial Resource and Application, Ministry of Agriculture/Department of Microbiology and Immunology, College of Biological Sciences, Agricultural University, China. Departamento de Microbiologı´a, Escuela Nacional de Ciencias Biolo´gicas, Instituto Polite´cnico Nacional, Mexico.
Subjects: Plant Production
Crop Improvement
Divisions: General
Depositing User: Mr Arbind Seth
Date Deposited: 20 Feb 2013 11:33
Last Modified: 20 Feb 2013 11:33
Official URL: 10.1016/j.syapm.2008.04.004

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