Overexpression of Xanthomonas campestris pv. vesicatoria effector AvrBsT in Arabidopsis triggers plant cell death, disease and defense responses

Hwang, I.N. and Kim, N.H. and Choi, D.S. and Hwang, B.K. (2012) Overexpression of Xanthomonas campestris pv. vesicatoria effector AvrBsT in Arabidopsis triggers plant cell death, disease and defense responses. Planta, 236 (4). pp. 1191-1204.

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Recognition of bacterial effector proteins by plant cells is crucial for plant disease and defense response signaling. The Xanthomonas campestris pv. vesicatoria (Xcv) type III effector protein, AvrBsT, is secreted into plant cells from Xcv strain Bv5-4a. Here, we demonstrate that dexamethasone (DEX): avrBsT overexpression triggers cell death signaling in healthy transgenic Arabidopsis plants. AvrBsT overexpression in Arabidopsis also reduced susceptibility to infection with the obligate biotrophic oomycete Hyaloperonospora arabidopsidis. Overexpression of avrBsT significantly induced some defense-related genes in Arabidopsis leaves. A high-throughput in planta proteomics screen identified TCP-1 chaperonin, SEC7-like guanine nucleotide exchange protein and calmodulin-like protein, which were differentially expressed in DEX:avrBsT-overexpression (OX) Arabidopsis plants during Hp. arabidopsidis infection. Treatment with purified GST-tagged AvrBsT proteins distinctly inhibited the growth and sporulation of Hp. arabidopsidis on Arabdiopsis cotyledons. In contrast, DEX:avrBsT-OX plants exhibited enhanced susceptibility to Pseudomonas syringae pv. tomato (Pst) DC3000 infection. Notably, susceptible cell death and enhanced electrolyte leakage were significantly induced in the Pst-infected leaves of DEX:avrBsT-OX plants. Together, these results suggest that Xcv effector AvrBsT overexpression triggers plant cell death, disease and defense signaling leading to both disease and defense responses to microbial pathogens of different lifestyles.

Item Type: Article
Additional Information: This work was supported by the Next Generation BioGreen21 Program (Plant Molecular Breeding Center; Grant No. PJ008027), Rural Development Administration, Republic of Korea.
Uncontrolled Keywords: Effector protein , AvrBsT , Pathogens , Disease , Defense responses , Cell death , Transgenic , Arabidopsis
Author Affiliation: Boyce Thompson Institute for Plant Research, Tower Road, Ithaca, NY 14853-1801, USA
Subjects: Plant Protection
Plant Physiology and Biochemistry > Biochemistry
Divisions: General
Depositing User: Mr Balakrishna Garadasu
Date Deposited: 20 Feb 2013 11:06
Last Modified: 20 Feb 2013 11:06
Official URL: http://dx.doi.org/10.1007/s00425-012-1672-4
URI: http://eprints.icrisat.ac.in/id/eprint/9648

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