Nucleotide sequence and genome organization of peanut stripe potyvirus

Gunasinghe, U.B. and Flasinski, S. and Nelson, R.S. and et al, . (1994) Nucleotide sequence and genome organization of peanut stripe potyvirus. Journal of General Virology, 75 (9). pp. 2519-2525.

[img] PDF - Published Version
Restricted to ICRISAT researchers only

Abstract

A blotch isolate of peanut stripe potyvirus (PStV) was cloned, sequenced and compared with other full-length potyvirus sequences. The viral genome was 10 059 nucleotides (nt) in length excluding the poly(A) tail. Two potential AUG start codons were identified in the 5′ non-translated region. Analysis of in vitro translation products from transcripts containing the first 600 nt of the PStV genome indicated that the first AUG (nt 134 to 136) was preferred over the second AUG (nt 146 to 148) for initiation of translation. Within the single large open reading frame, 8 processed proteins were predicted. In general, motifs conserved in other potyviral sequences were also found in the PStV genome. The presence of a 6K protein between the P3 and CI proteins was predicted. An altered amino acid motif, from FI (V) VRG to FMIIRG, within the carboxyl terminus of the P1 protein, separates the PStV sequence from the majority of potyvirus sequences. Based on comparisons with available full-length potyvirus genome sequences, PStV was found to be most closely related to soybean mosaic potyvirus.

Item Type: Article
Additional Information: Dr S N Nigam research collection - Box No:9
Uncontrolled Keywords: Nucleotide sequence, Genome Organization, Peanut stripe virus
Author Affiliation: Plant Biology Division, The Samuel Roberts Noble Foundation, USA.
Subjects: Crop Improvement > Genetics
Divisions: Groundnut
Depositing User: Mr Arbind Seth
Date Deposited: 29 Mar 2013 05:24
Last Modified: 29 Mar 2013 05:24
Official URL: http://dx.doi.org/10.1099/0022-1317-75-9-2519
URI: http://eprints.icrisat.ac.in/id/eprint/9998

Actions (login required)

View Item View Item